Overlay histogram showing Jurkat cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1-¦g/1*106cells) for 1 h at 4-¦C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4-¦C. Isotype control antibody (green line) was mouse IgG2b (1-¦g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Overlay histogram showing Raji cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1-¦g/1*106cells) for 1 h at 4-¦C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4-¦C. Isotype control antibody (green line) was mouse IgG2b (1-¦g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot

Western Blot